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A novel two-photon direct laser writing-based hybrid strategy for 3D nanoprinting microfluidic vessels with sophisticated 3D architectures and custom-designed micropores. 

Abstract The Sc2.0 global consortium to design and construct a synthetic genome based on theSaccharomyces cerevisiaegenome commenced in 2006, comprising 16 synthetic chromosomes and a new-to-nature tRNA neochromosome. In this paper we describe assembly and debugging of the 902,994-bp syntheticSaccharomyces cerevisiaechromosomesynXVIof the Sc2.0 project. Application of the CRISPR D-BUGS protocol identified defective loci, which were modified to improve sporulation and recover wild-type like growth when grown on glycerol as a sole carbon source when grown at 37˚C. LoxPsym sites inserted downstream of dubious open reading frames impacted the 5’ UTR of genes required for optimal growth and were identified as a systematic cause of defective growth. Based on lessons learned from analysis of Sc2.0 defects andsynXVI, anin-silicoredesign of thesynXVIchromosome was performed, which can be used as a blueprint for future synthetic yeast genome designs. Thein-silicoredesign ofsynXVIincludes reduced PCR tag frequency, modified chunk and megachunk termini, and adjustments to allocation of loxPsym sites and TAA stop codons to dubious ORFs. This redesign provides a roadmap into applications of Sc2.0 strategies in non-yeast organisms. 

The Technology Ambassador’s Program (TAP) was established in 2012 as an extra-curricular program and has been offered as a service learning course since spring 2016. To investigate the impact on program participants, we launched a longitudinal study in fall 2022 and surveyed the students who completed the course from spring 2016 to spring 2021. Analysis of the survey results discovered that students strongly agree that this program has provided them opportunities to conduct research, to network with other professionals in the field, and apply technical skills. Further analysis also revealed a strong correlation of these opportunities with improving soft skills and career readiness among participants. Overall, this program increased the confidence of the students and prepared them to learn new skills on their own. This paper describes the overall structure of the service learning program and presents the details of this study including the process and survey results. 

This Tutorial Review highlights strategies for leveraging the micron-to-submicron-scale additive manufacturing technique, “direct laser writing”, to enable 3D microfluidic technologies. 

Diagnostic classification models (DCMs) have seen wide applications in educational and psychological measurement, especially in formative assessment. DCMs in the presence of testlets have been studied in recent literature. A key ingredient in the statistical modeling and analysis of testlet-based DCMs is the superposition of two latent structures, the attribute profile and the testlet effect. This paper extends the standard testlet DINA (T-DINA) model to accommodate the potential correlation between the two latent structures. Model identifiability is studied and a set of sufficient conditions are proposed. As a byproduct, the identifiability of the standard T-DINA is also established. The proposed model is applied to a dataset from the 2015 Programme for International Student Assessment. Comparisons are made with DINA and T-DINA, showing that there is substantial improvement in terms of the goodness of fit. Simulations are conducted to assess the performance of the new method under various settings. 

This study focuses on a series of long-lived atmospheric bores that persisted from midnight to the late afternoon on 10 June 2023 over the coastal and offshore regions of the Yellow Sea. These bores were sustained by a favorable trapping mechanism associated with the stability of the marine boundary layer and the presence of a low-level wind component perpendicular to the propagation direction of the bores. These bores aided in maintaining and enhancing convective systems offshore with outflows that reached inland and initiated new convective cells. These convective cells eventually organized to produce severe weather under favorable thermodynamic conditions. These findings underscore the unique atmospheric conditions over the Yellow/East China Sea that promote long-lived atmospheric bores during the day, which is a potential mechanism to cause severe convective systems over the coastal region of eastern China. The complex multiscale processes influencing the development of mesoscale convective systems during the mei-yu monsoon season over this region present unique challenges for their accurate representation especially in coarse-grid weather and climate models. Given the growing density of coastal populations, further research is needed into the frequency of bores generated by convection and their subsequent impact on coastal convection. Significance StatementThis study links atmospheric bores over the Yellow/East China Sea to the generation of severe convection over the eastern coast of China. These offshore bores persisted well into the daylight hours with far longer lifetimes than their inland counterparts. This longevity was supported by the presence of a stable marine boundary layer and prevailing southerly winds associated with the sea breeze. These offshore bores influenced severe coastal daytime convection both directly and indirectly. This study reveals the complex characteristics of convective systems along the eastern coast of China. These findings underscore the need to incorporate offshore and coastal atmospheric measurements into operational forecasting and research activities.